This human clinical study examined how the tripeptide KPV (the C-terminal portion of alpha-melanocyte-stimulating hormone) affects corneal epithelial wound healing and the role of nitric oxide in this process. The research investigated the mechanisms by which KPV influences corneal epithelial cell repair, with particular focus on nitric oxide signaling pathways. This human clinical evidence provides direct data on KPV’s effects on corneal wound healing in human subjects.